Souris anti-Humain IgG1 (Fc Region) Anticorps (HRP)

N° du produit ABIN457836

Aperçu rapide pour Souris anti-Humain IgG1 (Fc Region) Anticorps (HRP) (ABIN457836)

Antigène: IgG1

Clonalité: Monoclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB)

Clone: NI 132 (HP 6186)

Isotype: IgG1 kappa

Détail du produit

Épitope: Fc Region

Reactivité: Humain

Hôte: Souris

Specificité: The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG1, subclass specific

Attributs du produit: Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Purification: Purified

Immunogène: Highly purified monoclonal IgG1 isolated from pooled human serum.

Information d'application

Indications d'application: To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:125 to 1:8,000, in Western blotting from 1:250 to 1:10.000. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgG1

Classe de substances: Antibody